Immunological and immunohistochemical features of endometrial implantation factor in healthy patients of late reproductive age

Аbstract. Relevance. The number of women older reproductive age is steadily increasing, and repeated failures Assisted Reproductive Technologies programs during the transfer high-quality embryos indicate possibility disruption embryo implantation processes associated with impaired receptivity functionality endometrium. Morphological, immunological immunohistochemical changes in endometrium factor may be decisive for formation «implantation window» correction these improve outcomes a cohort patients age. aim study - to expand pathogenetic understanding violation ability healthy Materials Methods. A prospective sample 46 (group 1), aged 38 45 years an officially registered diagnosis infertility lasting no more than 4 years, successful gynecological obstetric history, who were about have their first IVF attempt, was conducted. examined according Order № 803n Ministry Health Russian Federation. Additionally, level peripheral blood melatonin, determination progesterone, estrogen, HLA-DR (MHC II), CD56 (NK cells), CD138, leukemia inhibiting receptors studied. Concentrations IL-6, IL-10, TGFß, VGEF determined cervical secretion, calculation pro-inflammatory index, as ratio IL-6/IL-10 cu TGFß1/VEGF. Statistical data processing performed using Statistica 10.0 application software package (StatSoft, Inc., USA). Results Discussion. In group age, there imbalance steroid secretory transformation against background relative hyperestrogenism, decrease reception own hormones melatonin signals disorder pineal pituitary control over ovarian cycling. There expression factor. Signs inactive chronic endometritis autoimmune component are monitored, confirmed by cytokine balance. predominance fibrosis angiogenesis increase TGFß1/VEGF highly resistant flow uterine arteries. Conclusion. Standard pre-gravidar preparation cannot compensate all factors that violate potential this requires development new complex techniques directly affect diversity ensure natural extinction order effectiveness programs.